Published on: Mar 3, 2016
Transcripts - Nanomicrobiology
a branch of Nano Science MUSAB BIN ABDUL QADIR P0935030NANOMICROBIOLOGYNANOMICROBIOLOGY
Introduction Nanotechnology: A term that refers to technological developments on the nanometre scale. Nanomedicine Nanoscience Nanotechnology is a vast and expanding field.
NANOSCIENCE • The Science of Tiny It’s not biology, physics orNANOSCIENCE chemistry. It’s all sciences Things. that work with the very • A part of science that small. studies small stuff.
Nanomicrobiology.Application of nanobiotechnolgyin microbiology termed as Nanomicrobiology.It is the exploration of microbialcells on the nanoscale.
Nanomicrobiology The field of microbiology can be divided into two fields: Microscopy- required for visualisation ) Molecular biology- genetic and protein structure of organism
Nanomicrobiology and Atomic Force Microscopy Nanomicrobiology, the nanoscale analysis of microbial cells using atomic force microscopy (AFM) is an exciting, rapidly evolving research field. Over the past decade, there has been tremendous progress in use of AFM to observe membrane proteins and live cells at high resolution.
Atomic Force Microscopy AFM- Atomic Force Microscopy- New frontier on visualising, investigating and manipulating structure on a minute scale. Unlike electron microscopy –Has high resolution images that can be obtained under physiological conditions.
Atomic Force Microscopy Can measure interaction forces on the piconewton range! – 1 piconewton = 10-12 N Bacteria have cell wall composed of many layers- possible to manipulate and see various interactions Can be used for bacterial interactions involving the outer layer used for recognition events between cells. Can also visualize individual protein sub-units.
Atomic Force Microscopy Can also be used with Viruses Stacking of virus DNA or RNA can be seen and capsid properties as well Virus particles can be imaged on the surface of their target cell. The overall stability of this technology can be integrated with other microscopes and be used simultaneously.
Nanopatch Alternative to a syringe/needle Array of nano-sized projections on a patch 5mm x 5mm in size 3364 densely packed projections Each projection is 65 to 110µm in length Made of solid silicon and sputter-coated with a thin 100nm layer of gold Dry coated with antigen or DNA payload (solubilizes when wet)
NanopatchHow does it work?•Applied to your skin•Projections penetrate into the upper epidermal layers ofyour skin•Once contact is made with your skin the vaccine solubilizesWhy would we use Nanopatch?•Believed to improve efficacy and efficiency of vaccinationAdvantages of Nano Patch: Needle phobia Pain-free Cheap Prevent spread of infectious diseaseCurrent Research•Nanopatch-Targeted Skin Vaccination against West Nile Virus andChikungunya Virus in Mice•Designing a Nanopatch to match the spatial arrangements of APCs onskin.
Silver NanoparticlesWhat are Silver Nanoparticles? Tiny silver particles 1-100nm Toxicity towards bacteria is size dependant Most effective when 1-10nm in sizeHow does it work? Silver tends to have a higher affinity to react with phosphorus and sulfur compounds Binds to sulfur-containing proteins in the cell membrane, cellular proteins and phosphorous containing compounds (i.e. DNA) Leads to morphological distortion of cells and impairment of bacterial cycles, resulting in cell death
Nanomicrobiology Applications in Energy production
Nanomicrobiology Applications Production of energy fuels by microbial conversions along with renewable energy sources can literally replace the need for oil. Microorganisms use raw organic materials to make fuel. Can be used to yield- ethanol, hydrogen, methane, lipids, and butanol.
Nanomicrobiology Applications Through bioreactors – bacteria transform the chemical energy directly into electrical energy. Microbial redox systems along with nanotechnology such as nano scale wires or tubes can be utilized for electron transport. Microbial energy conversion advantage over petroleum and natural gas-based energy: environmentally friendly than fossil fuel technologies.
Nanotechnology also encompasses many other fields: Medicine Robotics Genetics Computer science Etc... The future is nano!